| Gene name: | MIR19B1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Hela,MCF-7 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Further investigation of miR-19b's effect on cell cycle arrest revealed that overexpression of exogenous miR-19b caused exiting from the G1 phase and entry into S or G2/M phase in both Hela and MCF7 cells .β-galactosidase staining showed that cellular senescence was reduced when miR-19b was overexpressed in both Hela cells and MCF7 cells, but increased 50% when miR-19b was silenced or p53 was overexpressed simultaneously. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | miR-19b was transfected into Hela cells and p53 levels of both transcriptional and translational were examined after 48 h. Western blot and qRT-PCR showed that p53 protein levels decreased by 50%, while mRNA levels were not altered. Similarly, the decrease of endogenous p53 protein was observed in both MCF7 and Huh7 cell lines when miR-19b was overexpressed, while mRNA level remained unchanged. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...