| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116,RKO |
| Gene ID: | 407040 |
| Category: | ncRNA |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 17875987 |
| Experiment: | SA-β-gal activity assay//Cell proliferation assay |
| Description: | We observed that introduction of miR-34a in HCT 116 cells caused senescence-like phenotypes with positive staining for senescence-associated β-galactosidase (SA-β-gal) and enlarged cellular size.RKO cells also showed similar morphological changes with enlarged cellular size by miR-34a introduction,although few SA-β-gal-positive cells were observed.The introduction of miR-34a caused a remarkable inhibition of cell proliferation in both HCT 116 and RKO cells compared with that of control miRNA. |
| Regulatory pathway: | E2F//P53 |
| R-AG-Pathway: | Downregulation//Upregulation |
| Official symbol(s): | E2F//TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | We also observed that p53 and p21 started to accumulate at 2 and 4 h, respectively, and the accumulation continued until 48 h after treatment.As expected, LoVo and RKO cells increased expression of miR-34a similar to HCT 116 cells, but DLD1 and HT29 cells showed no change, like the HCT 116 p53/ cells. Accumulation of p53 and p21 was observed in HCT 116, LoVo, and RKO cells, whereas HCT 116 p53/ cells showed no accumulation, and DLD1 and HT29 cells expressing mutant p53 showed consistent levels of p53 and no accumulation of p21.As for the down-regulated genes, E2F1, E2F2, and some E2F-target genes, including DHFR, MCM3, and MCM10, were observed among the list.introduction of miR-34a decreased the accumulation of E2F-1 and -3. E2F-2 was not detectable in either case . |
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