| Gene name: | MIR155 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//qPCR//Western blot |
| Description: | Determination of cellular senescence of HUVECs was conducted using 2 indicators– anti-SMP-30 and SA β-Gal staining – and TNF-α significantly reduced SMP-30 protein expression by almost 50% and enhanced SA β-Gal staining by nearly 2-fold. Then, the role of miR-155 in senescence of HUVECs was assessed, and the results showed that miR-155 mimic transfection increased SA β-Gal staining and downregulated SMP-30 .Addition of the miR-155 inhibitor decreased SA β-Gal staining and increased SMP-30 protein levels. |
| Regulatory pathway: | SIRT1-FOXO1-P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | SIRT1-FOXO1-TP53-CDKN1A |
| Pathway experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Pathway description: | Thus, the downstream signaling targets of SIRT1, including the protein levels of acetylated FoxO-1, acetylated p53, and p21, were also determined. Knockdown of miR-155 decreased p21, Ac-p53, and Ac-FoxO-1 protein expression, and SIRT1 silencing reversed this effect.Then, we assessed whether HUVEC proliferation and senescence were influenced by siSIRT1, and the results showed that siSIRT1 prevented the decrease in TNF-α-induced cell proliferation triggered by miR-155. Similarly, the effect of miR-155 on the upregulation of senescence and SMP-30 expression were substantially decreased by SIRT1 knockdown. |
Annotation:
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