| Gene name: | MIR101-1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SPAC-1-L,HEC-50 |
| Gene ID: | 406893 |
| Category: | ncRNA |
| Phenotype: | Endometrial cancer |
| Experimental category: | HL |
| PMID: | 25153722 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Introduction of miR-101 in SPAC-1-L and HEC-50 cells caused senescence-like phenotypes, such as positive staining for SA-β-gal and enlarged, flattened cell morphology. Furthermore, immunoblot analysis revealed that miR-101 overexpression markedly enhanced the expression of pro-apoptotic gene Bax, apoptosis marker cleaved-PARP and senescence marker p21 in either cell line. |
| Target gene: | EZH2//MCL-1//FOS |
| Official symbol(s): | EZH2//MCL-1//FOS |
| R-AG-Target gene: | Downregulation//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//qRT-PCR//Western blot |
| Target gene description: | In agreement with this, a negative correlation between endogenous miR-101 levels and EZH2, MCL-1 and FOS mRNA expression was found in EC cells .Next, we confirmed that endogenous mRNA and protein levels of these genes were downregulated in 101-transfected SPAC-1-L cells . Knockdown of miR-101 by AS-101 conversely induced the mRNA and protein levels of EZH2, MCL-1 and FOS in HOUA-I cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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