| Gene name: | MIR1258 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | H1299 |
| Experiment: | SA-β-gal activity assay |
| Description: | H1299 cells transfected with miR-1258 showed a strong blue SA-β-gal staining, while there were only scattered SA-β-gal positive cells in miR‐1258 inhibitor group. |
| Regulatory pathway: | GRB2-RAS-ERK |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | GRB2-KRAS-MAPK1 |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | GRB2 relative expression levels were down‐regulated with miR‐1258‐down‐regulated expression of si‐GRB2. Western blotting results showed that the expression levels of p‐c‐Raf, p‐Mek1/2 and p‐Erk1/2 were significantly decreased when miR‐1258 was overexpressed. However, the expression levels of p‐c‐Raf, p‐Mek1/2 and p‐Erk1/2 were significantly increased in cells with low expression levels of miR‐1258. There was no significant difference in Ras, c‐Raf, Mek1/2 and Erk1/2 expression in either case. These results indicate that miR‐1258 may be involved in the regulation of the GRB2/Ras/Erk pathway. |
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