| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ECa-109 |
| Gene ID: | 407040 |
| Category: | ncRNA |
| Phenotype: | Endometrial cancer |
| Experimental category: | L |
| PMID: | 26523671 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | After 4 days of incubation with miR-34a, we observed that the introduction of miR-34a in ECa-109 cells caused senescence-like phenotypes in most of the cells with positive staining for senescence associated β-galactosidase (SA-β-Gal), and cell number decreased with enlarged cellular size and morphological changes compared to the control group . |
| Target gene: | SIRT1//P53//P21 |
| Official symbol(s): | SIRT1//P53//P21 |
| R-AG-Target gene: | Downregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Western blot analysis revealed that the introduction of miR-34a (20 nM) decreased the expression of SIRT1 and increased the expression of p53 and p21 in ECa-109 cells; |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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