| Gene name: | MIR195 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | H1299,H1993,H358 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We observed significantly more cells in the G1 phase after miR-195 transfection, suggesting that miR195 causes arrest of NSCLC cells in the G1 phase.We found that miR-195 significantly increases the number of senescent cells, as measured by β-galactosidase staining assay . |
| Target gene: | CCND3//BIRC5 |
| Official symbol(s): | CCND3//BIRC5 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot//qRT-PCR |
| Target gene description: | We confirmed that mRNA and protein levels of CCND3 and BIRC5 are decreased by miR-195 and increased by miR-195 inhibitors in NSCLC cells.BIRC5 is predicted to contain one miR-195 target site in its 3′UTR, while CCND3 is predicted to contain two. We confirmed direct and specific regulation of BIRC5 and CCND3 by miR-195 using a luciferase reporter assay.We demonstrated that miR-195 represses CCND3 and BIRC5 proteins in a pair of cell lines derived from the same patient, HBEC30-KT and HCC4017 . |
| Regulatory pathway: | RB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Western blot |
| Pathway description: | We also found decreased levels of p-Rb by miR-195, indicating both activation of the retinoblastoma (Rb) pathway and senescence . |
Annotation:
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