| Gene name: | MIR338 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Brain |
| Cell name: | U251 |
| Gene ID: | 442906 |
| Category: | ncRNA |
| Phenotype: | Malignant glioma |
| Experimental category: | HL |
| PMID: | 30225541 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | When compared with the blank group and the NC group, the cells were found relatively large in size with high degree of senescence in the miR-338-5p mimic group and the siRNA-FOXD1 group, while were small in size with low degree of senescence in the miR-338-5p inhibitor group.When compared with the blank group and the NC group, the G0/G1 phase lengthened (increased proportion of cells), but the S phase shortened (decreased proportion of cells) in the miR-338-5p mimic group and the miR-338-5p mimic group. |
| Regulatory pathway: | FOXD1//MAPK |
| R-AG-Pathway: | Downregulation//Downregulation |
| Official symbol(s): | FOXD1//MAPK |
| Pathway experiment: | qRT-PCR//Western blot |
| Pathway description: | When compared with the control group, the miR338-5p expression and the mRNA and protein expression of Bax in cells of each group decreased significantly, and the protein and mRNA expression of FOXD1, MEK-2, ERK-1, Bcl-2, DAF, and PCNA increased obviously (p<0.05). |
Annotation:
Loading,please wait...