| Gene name: | MIR449B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SNU638 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Compared to negative control microRNAs, re-introduction of miR-449b strongly affected the proliferation of SNU638 cells and visual inspection of the cells indicated induction of apoptosis and cellular senescence. Flow cytometric analysis of propidium iodide-stained cells transfected with miR-449b showed a G1accumulation 48 hours after transfection, followed at 72 hours post transfection by an accumulation of cells in the sub G1 fraction suggestive of cell death.The induction of cellular senescence was confirmed by acidic beta gal staining using miR-34a as a positive control microRNA. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Western blot analysis showing an increase of the p53 protein upon miR-449 and positive control miR-34a re-introduction into SNU638 cells compared to RNA scrambled control as well as an activation of the p53 downstream target p21 and apoptosis markers cleaved CASP3 and PARP. |
Annotation:
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