Gene name: | MALAT1 |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | GBC-SD,SGC-996 |
Gene ID: | 378938 |
Category: | ncRNA |
Phenotype: | Gallbladder cancer |
Experimental category: | HL |
PMID: | 31174563 |
Experiment: | SA-β-gal activity assay//EdU assay//Transwell assay//Western blot |
Description: | Our results indicated that when MALAT1 was silenced, the viability of the GBC-SD and SGC-996 cells was suppressed, and the expression of Ki67 and PCNA was reduced. Meanwhile, the capabilities of migration and invasion were inhibited, while the SA-β-gal activity was promoted (p?0.05). |
Target gene: | ABI3BP//EZH2 |
Official symbol(s): | ABI3BP//EZH2 |
R-AG-Target gene: | Downregulation//-- |
Subcategory: | Methylation |
Target gene experiment: | qRT-PCR//Pull-down assay |
Target gene description: | We carried out the correlation analysis and found that the expression of MALAT1 and ABI3BP was negatively correlated.The binding of MALAT1 to EZH2 was further verified by RNA pull down. The results demonstrated that Bio-MALAT1-WT pull-down more EZH2 relative to that of the Bio-probe and Bio-MALAT1-MUT, suggesting that MALAT1 promoted the enrichment of EZH2. Higher expression of ABI3BP was identified among cells treated with sh-MALAT1 than that in sh-NC.Taken together, a conclusion was drawn that MALAT1 inhibited the expression of ABI3BP by recruiting EZH2 to the promoter region of ABI3BP. |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
Loading,please wait...