| Gene name: | HOTAIR |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38,IDH4 |
| Experiment: | qRT-PCR//SA-β-gal activity assay//Flow cytometry |
| Description: | The levels of senescent markers p53, p21 and p16 were elevated, and cells displayed the characteristic senescence- associated (SA) increase in G1 cell cycle compartment.That the increased HOTAIR was important for the implementation of senescence was evidenced by experiments in which silencing HOTAIR in IDH4 cells reversed the senescent phenotype, with a lower proportion of cells displaying the hallmark SA-β-galactosidase activity along with reduced levels of senescence markers. |
| Target gene: | ATAXIN-1//SNURPORTIN-1 |
| Official symbol(s): | ATAXIN-1//SNURPORTIN-1 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Co-IP |
| Target gene description: | Interestingly,the interaction of Dzip3 and Ataxin-1, as well as the interaction of Mex3b and Snurportin-1, as assessed by co-IP followed by western blot (WB) analysis, was facilitated by HOTAIR overexpression; furthermore, the levels of Ataxin-1 and Snurportin-1 decreased after HOTAIR overexpression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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