| Gene name: | MIR138-1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HuH-7,HepG2,Hepatocyte |
| Gene ID: | 406929 |
| Category: | ncRNA |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 28258280 |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | Telomeric repeat amplification protocol (TRAP) assay, senescence-associated β-galactosidase assay and BrdU incorporation assay were performed 24 and 48 h after transfection. The results showed that miR-138 functioned to decrease telomerase activity, which in turn induced cell senescence and suppressed cell proliferation. |
| Target gene: | TERT |
| Official symbol(s): | TERT |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR//Luciferase reporter assay |
| Target gene description: | The binding site of miR-138 in the 3’UTR of TERT gene was predicted by miRNA target prediction tools. The results showed that wild-type miR-138, but not mutant miR-138 (mutated in the seed-complementary sequence),can significantly suppress the luciferase activities of Huh7 cells transfected with wild-type TERT 3’UTR construct,Quantitative real-time PCR and Western blotting analysis showed that wild-type miR-138 mimic can suppress endogenous TERT mRNA and protein expression in Huh7 and HepG2 cells. These results indicated that TERT can be a direct target of miR-138 in HCC cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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