| Gene name: | MIR146A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HTM636,HTM1073 |
| Experiment: | SA-β-gal activity assay//BrdU assay//Flow cytometry |
| Description: | Production of SA-β-gal and iROS was significantly decreased, and BrdU incorporation was significantly increased by miR-146a in both HTM cell lines. |
| Target gene: | PAI-1//IRAK1 |
| Official symbol(s): | SERPINE1//IRAK1 |
| R-AG-Target gene: | Inhibition//-- |
| Subcategory: | Unclear |
| Target gene experiment: | BrdU assay |
| Target gene description: | We investigated whether the observed effects of miR-146a on SA-β-gal activity, iROS, and cell proliferation in senescent HTM cells were mediated by the downregulation of either the direct target IRAK1 or the secondary target PAI-1.Overexpression of both PAI-1 and IRAK1, lacking the miR-146a target site, inhibited the effects of miR-146a on cell proliferation.Among the different proteins repressed by miR-146a, the inhibition of PAI-1 may act to minimize the effects of senescence on the generation of iROS and growth arrest and prevent alterations of the extracellular proteolytic activity of the TM. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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