| Gene name: | MIR21 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | SA-β-gal activity assay//Knockdown//MTT assay//ELISA |
| Description: | Pre-miR-21 stimulated MCF-7 cell proliferation at the time points considered. In contrast, significant growth inhibition by ATRA was observed upon miR-21 silencing, indicating sensitization of MCF-7 cells. The effect of miR-21 silencing on ATRA-induced senescence was evaluated, using two molecular markers: -galactosidase and trimethyl K9 histone H3. |
| Target gene: | IL1B//ICAM-1//PLAT |
| Official symbol(s): | IL1B//ICAM1//PLAT |
| R-AG-Target gene: | Downregulation//--//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//qRT-PCR//Western blot |
| Target gene description: | We cloned the 3-UTR of the selected transcripts downstream of a luciferase reporter and evaluated the effect of miR-21 in 293T cells, which contain low levels of the miRNA (54) .MiR-21 inhibited the expression of the ICAM-1, PLAT, and IL1B constructs, indicating that they are direct targets.Forced expression of pre-miR-21 reduced ATRA-dependent induction of PLAT and IL1B mRNAs and proteins. By converse, the mRNA or protein expression pattern of ICAM-1 observed in ATRA-treated MDA-MB-231 cells was not affected by pre-miR-21 transfection. In contrast, pre-miR-21 enhanced the down-regulation of maspin mRNA and protein afforded by ATRA. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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