| Gene name: | LNCTAM34A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Colon |
| Cell name: | HCT116 |
| Experiment: | SA-β-gal activity assay//Knockdown//Cell viability assay |
| Description: | In addition, depletion of GUARDIN triggered cellular senescence.The effects of GUARDIN on cell viability were mirrored in the long-term survival of HCT116 cells in clonogenic assays and in their growth in nu/nu mice. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot//Cell viability assay//Knockdown |
| Pathway description: | Although GUARDIN knockdown in the absence of p53 did not significantly affect cell viability, cell death was enhanced following induction of p53 . Conversely, overexpression of GUARDIN diminished the reduction in cell viability associated with p53 induction. Thus, although p53 controls the function of GUARDIN through regulating its expression, GUARDIN modulates the cytotoxic effect of p53.We extended our analysis to multiple lines, confirming that overexpression of wild-type p53 in U2OS osteosarcoma, A549 lung adenocarcinoma and HCT116 cells increased GUARDIN levels, whereas knockdown of p53 decreased GUARDIN expression. |
Annotation:
Loading,please wait...