| Gene name: | MIR675 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | H9C2 |
| Gene ID: | 100033819 |
| Category: | ncRNA |
| Phenotype: | Vascular disease |
| Experimental category: | L |
| PMID: | 30889706 |
| Experiment: | SA-β-gal activity assay//EdU assay |
| Description: | The data showed that miR-675 mimic increased the expression of miR-675 and miR-675 inhibitor decreased the expression of miR-675.Importantly, the miR-675 mimic decreased β-gal staining and increased the numbers of proliferating cells, while the miR-675 inhibitor showed an opposite effect on β-gal staining and cell proliferation. |
| Regulatory pathway: | TGFβ1-P21 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TGFB1-CDKN1A |
| Pathway experiment: | Luciferase reporter assay//qRT-PCR//Western blot |
| Pathway description: | Dual-luciferase reporter assays showed that the miR-675 mimic decreased the luciferase activity compared to the control. To confirm that TGF-β1 is a target of miR675 in H9C2 cells, we transfected miR-675 mimic or inhibitor into these cells. We found that miR-675 mimic and inhibitor did not alter the expression of TGF-β1 mRNA, but miR-675 mimic decreased the expression level of TGF-β1 protein and miR-675 inhibitor has the opposite effect. These findings verified TGF-β1 as the target gene of miR-675. Moreover, p21 protein level was reduced by miR-675 mimic, but increased by miR-675 inhibitor. |
Annotation:
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