| Gene name: | MIR15B |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ES‐2,Caov‐4,SKOV3,OVCAR-3,OV‐1063 |
| Experiment: | MTT assay//Flow cytometry//SA-β-gal activity assay |
| Description: | Cell proliferation, migration and invasion abilities in addition to cell proportion in the S phase in response to miR-15b mimic and siRNA LPAR3 significantly decreased while apoptosis and senescence, as well as cell proportion in G0/G1 phase significantly elevated (p < 0.05). |
| Target gene: | LPAR3 |
| Official symbol(s): | LPAR3 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Dual-Luciferase reporter assay |
| Target gene description: | The luciferase reporter exhibited that in comparison with the NC group, the luciferase activity of LPAR3 WT was profoundly reduced by miR 15b (p < 0.05), whereas that of the LPAR3 MUT was not changed. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | In relative to the blank and NC groups, the miR-15b and Bax expression in ovarian cancer cells treated with miR 15b mimic dramatically enhanced (p < 0.05), but that of LPAR3 and Bcl 2, as well as the extent of PI3K and Akt phosphorylation, remarkably diminished (p < 0.05). |
Annotation:
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