Aging Overview

Aging gene

Gene name: MIR1292
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: Adipose stromal cell
Gene ID: 100302138
Category: ncRNA
Phenotype: Age-related osteoporosis
Experimental category: L
PMID: 30574422
Experiment: qRT-PCR//Western blot//SA-β-gal activity assay
Description: qRT-PCR and western blot analyses also showed that lenti-1292-infected hADSCs expressed higher levels of the senescence markers P16 and P21 and lower expression of LMNB1 than control cells.Moreover, the number of SA-β-gal-stained cells was increased after miR-1292 overexpression.


Regulatory relationship

Target gene: FZD4
Official symbol(s): FZD4
R-AG-Target gene: Downregulation
Subcategory: Unclear
Target gene experiment: Luciferase reporter assay//qRT-PCR//Western blot
Target gene description: Results showed that miR-1292 overexpression remarkably inhibited luciferase reporter activity from the vector containing the WT FZD4 3′ UTR, but not from the mutated FZD4 or those of other genes. Additionally, after miR-1292 overexpression, FZD4 was markedly down -regulated at the protein level, but not at mRNA level, Also, qRT-PCR results showed that the expression of miR-1292 was negatively correlated with FZD4 in 70 clinical bone samples.

Regulatory pathway: WNT-β-CATENIN
R-AG-Pathway: Downregulation
Official symbol(s): Wnt-CTNNB1
Pathway experiment: Western blot
Pathway description: Western blotting studies revealed that miR-1292 overexpression decreased both FZD4 and β-catenin expression, indicating that Wnt/β-catenin signaling was impaired.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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