| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U87,MSC |
| Experiment: | qRT-PCR//CCK-8 assay//SA-β-gal activity assay//ELISA |
| Description: | Co-culturing with MSCs overexpressing miR-34a for 24 h impaired U87 glioma cell proliferation. Additionally, it induced expression of senescence-associated genes p53, Cdkn1a and Cdkn2c. As expected,this result was corroborated by an increase in the percentage of cells positively marked with the senescence-associated β-galactosidase. It was identified that co?culture with hMSCs overexpressing miR-34a decreased the telomere length and impaired the telomerase activity. |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | The results confirmed that the level of SIRT1 decreased in U87 cells co-cultured with hMSCs overexpressing miR-34a, while NC mimic transfection had no observable effect. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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