| Gene name: | MIR203A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay |
| Description: | The percentage of β-galactosidase staining-positive cells in the ox-LDL group was significantly increased compared to that in the control group. The ox-LDL-induced endothelial cell senescence was markedly reduced in the presence of miR-203a-3p inhibitor. The negative control of inhibitor did not exert effect on ox-LDL-induced endothelial cell senescence. |
| Target gene: | DRP1 |
| Official symbol(s): | DRP1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | Both mRNA and protein expressions of Drp1 were significantly downregulated in the ox-LDL-treated HUVECs, which were reversed in the presence of miR-203a-3p inhibitor. |
| Regulatory pathway: | AMPK-P53//AMPK-P16 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | PRKAA1-TP53//AMPK-CDKN2A |
| Pathway experiment: | Western blot |
| Pathway description: | The phosphorylation level of AMPK(p-AMPK) was obviously elevated in the ox-LDL-treated HUVECs compared to that in the control cells, concomitant with an up-regulation of p53 and p16, indicating an activation of AMPK-p53/p16 pathway.These phenomena were blocked by miR-203a-3p inhibitor. |
Annotation:
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