| Gene name: | MIR29C |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//EdU assay//CCK-8 assay//qRT-PCR |
| Description: | The results demonstrated that the over-expression of miR-29c-3p significantly enhanced the SA-β-gal staining compared with that of the negative control (NC) group. EdU was not incorporated into the SA-β-gal positive hMSCs, indicating the senescent hMSCs were indeed not proliferating.the CCK-8 assay was performed ,The results showed that the proliferative capacity of hMSCs was inhibited after the Agomir-29c-3p transfection.The qPCR results indicated that p16, p21, PTGS2, AKAP9, CCND1 and EDN1(senescence markers ) were significantly increased after miR-29c-3p transfection. |
| Target gene: | CNOT6 |
| Official symbol(s): | CNOT6 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | We transfected hMSCs with Agomir-29c-3p or mirVana-29c-3p and examined CNOT6 expression using qPCR and WB assays. The results showed that the CNOT6 mRNA level was decreased in the Agomir-29c-3p group, but increased in the mirVana-29c-3p group. |
| Regulatory pathway: | P53-P21//P16-PRB |
| R-AG-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53-CDKN1A//CDKN2A-RB1 |
| Pathway experiment: | Western blot//qRT-PCR |
| Pathway description: | Western blot (WB) results also suggested that the expression of p53, p21, p16, PTGS2 and pRB were all increased gradually. |
Annotation:
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