| Gene name: | MIR137 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Gene ID: | 406928 |
| Category: | ncRNA |
| Phenotype: | Pancreatic cancer |
| Experimental category: | L |
| PMID: | 26904954 |
| Experiment: | SA-β-gal activity assay//Immunostaining |
| Description: | A robust senescence response was observed upon transfection of miR-137 compared to miR-67-transfected cells, as assessed using senescence-associated β-galactosidase activity .an increase in the number of PML nuclear bodies and γH2AX foci, two known markers of cells undergoing cellular senescence, also were observed in miR-137-expressing cells. |
| Target gene: | KDM4A |
| Official symbol(s): | KDM4A |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | The mRNA level of CHD5, a transcriptional target of KDM4A (Mallette and Richard, 2012), was increased following transfection with miR-137, consistent with decreased KDM4A mRNA expression and protein levels. |
| Regulatory pathway: | ARF-P53//P16-PRB |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | CDKN2A-TP53//CDKN2A-RB1 |
| Pathway experiment: | SA-β-gal activity assay//qRT-PCR |
| Pathway description: | Expression of human papilloma viral E6 and E7 in miR-137-transfected fibroblasts fully rescued the senescence phenotype, suggesting that miR-137 indeed relies on either the p53 or p16INK4A?pathway to establish senescence. |
Annotation:
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