| Gene name: | OVAAL |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ME4405,HCT116 |
| Gene ID: | 148756 |
| Category: | ncRNA |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 30478051 |
| Experiment: | Knockdown//PI staining//Flow cytometry//SA-β-gal activity assay |
| Description: | OVAAL shRNA inhibited cancer cell proliferation, which was substantiated through G0/G1 cell cycle arrest and decreased phosphorylated Rb (p-Rb); depletion of OVAAL also triggered cellular senescence as shown by induction of senescence-associated (SA)–?-gal staining . |
| Target gene: | STK3//P27 |
| Official symbol(s): | STK3//P27 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qPCR//RIP |
| Target gene description: | Further examination of the relationship between OVAAL and p27 expression showed that the increased p27 protein levels observed after silencing of OVAAL did not increase p27 mRNA levels.Consistently, OVAAL was coimmunoprecipitated by STK3 antibodies by RNA immunoprecipitation (RIP) assays in ME4405 and HCT116 cells . |
| Regulatory pathway: | RAF-MEK-ERK |
| R-AG-Pathway: | Activation |
| Official symbol(s): | ZHX2-MAP2K7-MAPK1 |
| Pathway experiment: | Western blot |
| Pathway description: | Silencing of OVAAL prominently reduced the activation of RAF/MEK/ERK cascade as shown by decreases in p-MEK, p-ERK, p-MSK1, and p-RSK1, recapitulating the effects observed after knockdown of STK3 in ME4405 and HCT116 cells. |
Annotation:
Loading,please wait...