Aging Overview

Aging gene

Gene name: OVAAL
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: ME4405,HCT116
Gene ID: 148756
Category: ncRNA
Phenotype: Colorectal cancer
Experimental category: L
PMID: 30478051
Experiment: Knockdown//PI staining//Flow cytometry//SA-β-gal activity assay
Description: OVAAL shRNA inhibited cancer cell proliferation, which was substantiated through G0/G1 cell cycle arrest and decreased phosphorylated Rb (p-Rb); depletion of OVAAL also triggered cellular senescence as shown by induction of senescence-associated (SA)–?-gal staining .


Regulatory relationship

Target gene: STK3//P27
Official symbol(s): STK3//P27
R-AG-Target gene: --//--
Subcategory: Unclear
Target gene experiment: Western blot//qPCR//RIP
Target gene description: Further examination of the relationship between OVAAL and p27 expression showed that the increased p27 protein levels observed after silencing of OVAAL did not increase p27 mRNA levels.Consistently, OVAAL was coimmunoprecipitated by STK3 antibodies by RNA immunoprecipitation (RIP) assays in ME4405 and HCT116 cells .

Regulatory pathway: RAF-MEK-ERK
R-AG-Pathway: Activation
Official symbol(s): ZHX2-MAP2K7-MAPK1
Pathway experiment: Western blot
Pathway description: Silencing of OVAAL prominently reduced the activation of RAF/MEK/ERK cascade as shown by decreases in p-MEK, p-ERK, p-MSK1, and p-RSK1, recapitulating the effects observed after knockdown of STK3 in ME4405 and HCT116 cells.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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