| Gene name: | MIAT |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry//Western blot |
| Description: | The results showed an increase in the proportion of cells in G1 phase and a decreased in the proportion of cells in G2/M phase in Miat knock down cells.The result revealed that Cyclin D1 expression level was downregulated in cells treated with Miat siRNA in comparison to cells treated with scramble siRNA, which indicated Miat inhibition caused cell cycle arrest. The result revealed that the proportion of senescent cells was dramatically elevated in Miat knockdown cells in comparison to control cells. Consistent with above stain positive for senescence-associated β-galactosidase activity, the expression of cellular senescent biomarkers, p16Ink4A and Cox2, markedly increased following Miat suppression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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