| Gene name: | MIR21 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay//MTT assay//qPCR |
| Description: | Stable miR21 over-expression indeed significantly reduced cell proliferation consistent with the transient over-expression results above.Cell growth was further monitored during the entire replicative lifespan, and indeed, miR-21 over-expressing cells underwent replicative senescence earlier than control cells.Furthermore, miR-21 over-expression led to an increase in senescent cells, as the majority of cells stain positive for senescenceassociated ?-galactosidase (SA ?-gal) at PD1pT after selection of stable cells. |
| Target gene: | CDC25A//NF1B |
| Official symbol(s): | CDC25A//NF1B |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | CDC25A and NF1B are direct targets of miR‐21. The expression of a luciferase reporter gene containing the CDC25A 3′‐UTR (A) or NF1B 3′‐UTR (B), respectively, was suppressed by miR‐21 in HUVEC. The suppression is specific to the miR‐21 seed region within the CDC25A 3′‐UTR or NF1B 3′‐UTR, as mutation of the miR‐21 target sites alleviated repression by miR‐21. Ratios of CDC25A‐ or NF1B‐dependent renilla luciferase signal (rLuc) to a firefly luciferase signal (fLuc) encoded by the same vector are given. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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