| Gene name: | MIR449A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | B5,PC-3,DU 145,DU-1.1 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We transfected DU-145 sublines with miR-449a and stained for SA-β-gal activity. At 10 and 25 nM concentrations, DU-1.1 and B5 cells stained positive for SA-β-gal, while staining in mock and miR-Con treatments were nearly undetectable . In PC-3 cells, miR-449a caused G0/G1 arrest as indicated by the increase in G0/G1 cell number and corresponding reductions in S and G2/M populations. |
| Target gene: | CYCLIN D1//HDAC1 |
| Official symbol(s): | CYCLIN D1//HDAC1 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown |
| Target gene description: | miR-449a significantly reduced Cyclin D1 protein levels in PC-3 cells.Knockdown of HDAC1 by miR-449a or siHDAC1 elevated p27 protein and reduced P-Rb levels. |
| Regulatory pathway: | RB |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | retinoblastoma |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | Knockdown of Cyclin D1 by miR-449a or siCCND1 drastically reduced phophorylated Rb (P-Rb) levels. Knockdown of HDAC1 by miR-449a or siHDAC1 elevated p27 protein and reduced P-Rb levels. |
Annotation:
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