| Gene name: | MIR125B1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | KYSE150,KYSE510 |
| Gene ID: | 406911 |
| Category: | ncRNA |
| Phenotype: | Esophageal cancer |
| Experimental category: | HL |
| PMID: | 28968424 |
| Experiment: | CCK-8 assay//SA-β-gal activity assay//Transwell assay |
| Description: | Overexpression of miR-125b-5p significantly inhibited cell proliferation of KYSE150 and KYSE510 cell lines.Using Senescence β-Galactosidase Staining Assay, we found that overexpression of miR-125b-5p significantly enhanced the senescence of ESCC cells. We also found that miR-125b5p overerxpression significantly inhibited the migration and invasion of ESCC cells. |
| Target gene: | HMGA2 |
| Official symbol(s): | HMGA2 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR//Knockdown//Dual-Luciferase reporter assay |
| Target gene description: | HMGA2 was one of the candidate targets, and down-regulated by miR-125b-5p mimics both in mRNA and protein levels. Dual-luciferase reporter assays found that luciferase activity was significantly down-regulated in the miR-125b-5p mimics and pGL3-HMGA2-3’UTR-Wt group compared with NC group. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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