| Gene name: | MIR340 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | GIC |
| Experiment: | BrdU assay/Western blot//SA-β-gal activity assay//Flow cytometry |
| Description: | We observed that miR-340-overexpressing cells became flatter and larger than control cells.Immunohistochemical analysis and Western blot assays revealed that miR-340 overexpressing hGICs partially decreased Nestin expression and lost the expression of Sox2, but remained positive for GFAP, an astrocyte marker. miR-340-overexpressing hGICs ceased proliferating during the first 3 days of culture. The BrdU-incorporation and cell cycle analyses revealed that miR-340 overexpression significantly arrested the cell cycle at the G1/S transition, as indicated by a marked accumulation of cells in the G1 peak and by a reduction of cells in the S phase. We further demonstrated that miR-340 overexpression activated the expression of SA-β-gal, a marker of cellular senescence, in hGICs. |
| Target gene: | PLAT |
| Official symbol(s): | PLAT |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot//Immunohistology |
| Target gene description: | Immunohistochemical analysis and Western blot assays confirmed that miR-340 overexpression decreased PLAT expression in hGICs. Using a reporter vector encoding the firefly luciferase gene with the wild-type plat 3’UTR, we demonstrated that miR-340 overexpression inhibited luciferase activity in hGICs, whereas a deletion in the predicted binding site of miR-340 in the 3’UTR of the plat gene abrogated the aforementioned inhibitory effect of miR-340. Taken together, these data strongly indicate that PLAT is a novel direct target of miR-340 in hGICs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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