| Gene name: | MIR449A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 95D,A549 |
| Experiment: | EdU assay/SA-β-gal activity assay//Flow cytometry |
| Description: | MiR-449a introduction caused a remarkable inhibition of cell growth in both A549 and 95D cells relative to NC. The EdU incorporation assay also revealed that the growth of A549 and 95D cells was significantly inhibited 231 by miR-449a relative to NC . The EdU cell proliferation as- 232 say determined that miR-449a suppressed the entry of A549 and 233 95D cells into the S phase.The miR-449a mimics caused significant G0/G1 arrest in A549 and 95D cells. We also observed that miR-449a in A549 and 95D cells caused senescent phenotypes with positive staining for senescence-associated β-galactosidase (SA-β-gal) and led to drastic changes in cell morphology, including increased size and a broad, flattened shape 72 h following transfection. |
| Target gene: | E2F3 |
| Official symbol(s): | E2F3 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Dual-Luciferase reporter assay//qRT-PCR//Western blot |
| Target gene description: | The luciferase reporter assay indicated that the luciferase activity of the re porter containing the E2F3 gene’s wide-type 3' -UTR decreased (52%) following treatment with miR-449a mimics. By contrast, the inhibitory effect of the miR-449a mimics was abolished in the mutated construct. In addition, qRT-PCR and western blot analysis revealed that the expression of E2F3 mRNA and protein was inhibited by treatment with miR-449a mimics in A549 and 95D cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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