| Gene name: | MIR152 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDPSC |
| Experiment: | SA-β-gal activity assay//MTT assay |
| Description: | The MTT assay showed that miR152 upregulation significantly decreased the viability of HDPSCs.Furthermore, we found that miR-152 overexpression significantly increased the number of SA-β-gal-positive cells concomitant with a significant increase in p21 and p16 expression levels compared with vector control cells. |
| Target gene: | SIRT7 |
| Official symbol(s): | SIRT7 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//qRT-PCR//Luciferase reporter assay |
| Target gene description: | Transduction of late-passaged HDPSCs with a lentiviral vector encoding anti-miR-152 effectively downregulated miR-152 and upregulated SIRT7.Luciferase activity assays showed that miR-152 significantly decreased luciferase activity in cells transfected with the wild-type but not the mutant SIRT7 3'-UTR-binding site, confirming that SIRT7 is a direct target of miR-152. Real time RT-PCR and western blot analysis showed that SIRT7 was significantly downregulated in HDPSCs at PD54 compared with its expression at PD16, and significantly downregulated by ectopic expression of miR-152, further supporting SIRT7 as a direct target of miR-152. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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