| Gene name: | MIR34A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HASMC |
| Gene ID: | 407040 |
| Category: | ncRNA |
| Phenotype: | Cardiovascular disease |
| Experimental category: | L |
| PMID: | 25352462 |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry |
| Description: | In comparison with the control (miRNA mimic control [SCR]), miR-34a mimic reduced cell number already by 48 hours after transfection;the main and statistically significant differences were observed at 72- hours post-transfection. Further, we evaluated cell cycle distribution by fluorescence-activated cell sorting analysis after propidium iodide (PI) staining. miR-34a ectopic expression significantly increased the percentage of HASMCs in G0–G1 phase at 24-hours post-transfection when compared with negative control.Accordingly, p21 protein levels were higher in miR-34a-overexpressing cells.We found that miR-34a enhanced the percentage of SA-β-gal-positive cells at 72 hours after transfection, while HASMCs transfected with the negative control did not show any difference when compared with untransfected cells . |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We then performed a rescue experiment by transfecting replicative cells with miR-34a mimic or mimic negative control along with either a 3?-untraslated region-deleted-SIRT1-expression vector (devoid of the sequences matching miR-34a seed sequence) or the corresponding empty vector. The WB analysis confirmed that endogenous SIRT1 protein levels were severely lowered upon miR-34a overexpression, while exogenous SIRT1 protein levels were unaffected. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...