| Gene name: | MIR10A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | Down regulation of miR-10a expression increased the percentage of SA-β-gal positive cells among the LV-anti-10a-infected groups compared to the LV-controlinfected groups, indicating that repression of miR-10a increased hMSC senescence in both young and old hMSCs. |
| Target gene: | KLF4 |
| Official symbol(s): | KLF4 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot//qRT-PCR |
| Target gene description: | Luciferase activity was significantly repressed by the miR-10a mimic, proving the direct binding of the miR-10a to the 30 -UTR of KLF4. A similar effect was also observed in the hMSCs.To determine if miR-10a would affect endogenous KLF4 expression, we compared KLF4 expression after infecting the hMSCs with LV-miR-10a and LVanti-10a. Up regulation of miR-10a repressed both the endogenous mRNA and protein expression of KLF4. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...