| Gene name: | MIR107 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | Cell cycle analysis |
| Description: | Cell cycle analysis showed a significant increase in the percentage of cell population arrested at G0/G1 phase in the mimic miR-107 transfected cells compared to the mimic negative control cells.Moreover, the percentage of cell population in S-phase was also significantly lower in the mimic miR-107 transfected cells compared to the mimic negative control cells. |
| Target gene: | P16NK4A//PTEN |
| Official symbol(s): | P16NK4A//PTEN |
| R-AG-Target gene: | Upregulation//Downregulation |
| Subcategory: | Phosphorylation Unclear |
| Target gene experiment: | Western blot//RNA Pull-down assay//qRT-PCR |
| Target gene description: | Over-expression of miR-107 also resulted in significant augmentation of the expression of P16INK4A .For further verification, miRNA-mRNA complex pull-down assay was performed.Accordingly, qRT-PCR analysis demonstrated a significant enrichment of PTEN mRNA in the pull-down miRNA-mRNA complex . In addition, PTEN protein expression was down-regulated following the over-expression of miR-107 similar to that observed in the 1 nM rapamycin-treated pre-senescent HUVECs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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