| Gene name: | H19 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NPC |
| Gene ID: | 283120 |
| Category: | ncRNA |
| Phenotype: | Disc degenerative disease |
| Experimental category: | L |
| PMID: | 29520849 |
| Experiment: | SA-β-gal activity assay//Immunofluorescence |
| Description: | SA-β-Gal positive cells and Collagen I content were increased, while telomerase activity was decreased by H19 overexpression. |
| Target gene: | MIR-22 |
| Official symbol(s): | MIR-22 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//qPCR |
| Target gene description: | In NPCs, H19 and miR-22 negatively regulated each other. The results showed that the luciferase activity of wild-type vectors, wt-19 and wtLEF1 3 UTR could be suppressed by miR-22 mimics while amplified by miR-22 inhibitor; after mutating the predicted miR-22 binding site, the changes of luciferase activity were totally eliminated. |
| Regulatory pathway: | WNT-β-CATENIN |
| R-AG-Pathway: | -- |
| Official symbol(s): | Wnt-CTNNB1 |
| Pathway experiment: | Spearman's rank correlation analysis |
| Pathway description: | Furthermore, H19 expression was positively correlated Wnt3 and β-catenin expression in tissue specimens, respectively.The data indicate that H19 modulates Wnt signaling pathway through miR-22; miR-22 could partially reverse the effect of H19 on Wnt signaling pathway. |
Annotation:
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