| Gene name: | MIR23A |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | K562 |
| Gene ID: | 407010 |
| Category: | ncRNA |
| Phenotype: | Chronic myeloid leukemia |
| Experimental category: | HL |
| PMID: | 25213664 |
| Experiment: | SA-β-gal activity assay |
| Description: | Strongly positive β-gal staining was observed in K562 cells transfected with miR-23a precursors compared with controls and the difference was statistically significant. |
| Regulatory pathway: | BCR-ABL-PI3K-AKT-MMP9 |
| R-AG-Pathway: | -- |
| Official symbol(s): | BCR-ABL1-PI3K-AKT-MMP9 |
| Pathway experiment: | qRT-PCR//Western blot//Luciferase reporter assay |
| Pathway description: | Transfection with miR-23a precursor decreased the BCR/ABL 3′-UTR reporter activity in K562 cells, which strongly indicated that BCR/ABL was a target for miR-23a.Finally, we assessed the effect of miR-23a expression on BCR/ABL expression. Transfection of K562 cells with miR-23a precursor resulted in lower expression of BCR/ABL after 48h. Concomitant with decreased BCR/ABL expression, we observed reduction of its downstream targets PI3K, Akt and MMP-9, as we previously reported8. |
Annotation:
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