Aging Overview

Aging gene

Gene name: HOTAIR
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: A2780_CR5
Gene ID: 100124700
Category: ncRNA
Phenotype: Ovarian cancer
Experimental category: HL
PMID: 27041570
Experiment: SA-β-gal activity assay//PI staining//Flow cytometry//Knockdown
Description: In addition, IL-6 secretion, an established marker for cell senescence was increased by CDDP in A2780 p overexpressing HOTAIR compared with vector-transfected cells .DsiRNA knockdown of HOTAIR in A2780_CR5 cells reduced the number of senescent cells.We observe a decrease in S1 and an increase in G2 phase 48 h post CDDP treatment in HOTAIR expressing cells and a reversal of this effect in A2780_CR5 cells, suggesting that a subpopulation of cells undergo HOTAIR-dependent cell senescence.


Regulatory relationship

Regulatory pathway: NF-κB//CHK1-P53-P21
R-AG-Pathway: --//--
Official symbol(s): NFKB1//CHEK1-TP53-CDKN1A
Pathway experiment: Western blot//SA-β-gal activity assay//MTT assay
Pathway description: In HOTAIR overexpressing A2780p cells, SA-β-Gal-positive cell numbers were increased by high vs low evels of CDDP , and NF-κB inhibitor Bay-11 reduced the number of senescent cells,ctopic expression of HOTAIR in NF-κB knockdown cells rescued (Po0.05) proliferation and increased clonogenic survival.In HOTAIR overexpressing vs vector control cells, decreased p-p53 levels were observed and the level of p-Chk1 was essentially unchanged , suggesting ATR dependent activation of p53 by HOTAIR. Activation of p53 and p21 by HOTAIR was observed only during high CDDP treatment, indicated by p53 phosphorylation and p21 expression .


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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