| Gene name: | MIR186 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Gene ID: | 406962 |
| Category: | ncRNA |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 23137536 |
| Experiment: | SA-β-gal activity assay |
| Description: | To examine the role of miR-186, miR-216b, miR-337-3p, and miR-76 in cellular senescence, we knocked down CKIIa in HCT116 cells by co-treatment with the same miRNA mimics.To assess the effect of miRNA knockdown on senescence, after transfection for 2 days the transfectants were then stained for SA-β-gal activity. HCT116 cells transfected with the four mimics exhibited a higher rate of SA-β-gal staining compared with control cells . |
| Target gene: | CKII//P53//P21/WAF1 |
| Official symbol(s): | CSNK2A1//P53//P21/WAF1 |
| R-AG-Target gene: | Downregulation//Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | CKII activity assay//Western blot |
| Target gene description: | When CKII activity was assessed using [c32P]ATP and CKII peptide substrate, extract from the cells transfected with the four mimics contained lower than 40% CKII activity compared to control extract.Immunoblot data showed that the expression levels of p53 and p21Cip1/WAF1 were upregulated in HCT116 cells treated with the four miRNA mimics in comparison with control cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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