| Gene name: | MIR433 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A2780 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | Firstly,to investigate if miR-433 overexpression could induce senescence the protein levels of p16, phosphorylated Rb (p-Rb), and p21 were anaylzed in the miR-433-stable A2780 cells. This analysis showed that p-Rb was decreased in A2780 cells stably transfected with miR-433. Notably, there was no reciprocal upregulation of p16 and p21 in these cells . The senescence-associated β-galactosidase activity in these cells was determined by Western blot and β-galactosidase staining analyses and revealed a significant upregulation of senescence-associated β-galactosidase activity in miR-433-stable cells compared to controls(P < 0.001). |
| Target gene: | CDK6 |
| Official symbol(s): | CDK6 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | In our earlier bioinformatics analysis of potential miR-433 targets, CDK6 was predicted by five of the seven databases as a candidate miR-433 target gene. Therefore, we set out to establish if miR-433 could regulate the expression of CDK6. By analyzing protein expression in both the miR-433 stable A2780 cells and the clonal derivative of this cell line, we observed a decrease in CDK6 expression. Additionally, transient overexpression of miR-433 in HeLa cells also demonstrated downregulation of CDK6. Moreover, the transient transfection of PEO1 cells with anti-miR-433 to inhibit miR-433, resulted in a demonstrable upregulation of CDK6. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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