| Gene name: | MIR424 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Fibroblast |
| Experiment: | RT-PCR |
| Description: | RT-PCR analysis confirmed a gradual increase in the expression levels of each SA-miRNA with increase in population doublings in fibroblasts.In addition,transient overexpression of each individual SA-miRNAs in fibroblasts also reduced cell proliferation and increased p16 levels. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown |
| Target gene description: | Stable knockdown of p16(HMEC.p16shRNA) generated a relative increase in PcG member expression, particularly in the case ofCBX7. Concordantly, SA-miRNA levels were reduced.We examined the impact of the transient loss of p16 on PcG and SA-miRNA expression.Transient transfection of presenescent HMECs with a potent siRNA targeting p16 increased the expression of each PcG member and repressed SA-miRNA levels in HMEC to a great degree than the changes observed. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...