| Gene name: | MIR181A1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ULM,MM |
| Gene ID: | 406995 |
| Category: | ncRNA |
| Phenotype: | Uterine leiomyomas |
| Experimental category: | HL |
| PMID: | 30097674 |
| Experiment: | SA-β-gal activity assay |
| Description: | ULM and MM cells overexpressing the miRNAs and cultured as spheroids were fixed and stained for SA-β-galactosidase. Spheroid MM and ULM showed a global senescence pattern and a significant increase in cellular senescence was observed in both ULM and MM spheroids with overexpressing miR-29b, miR-181a, miR-182, and miR-200c compared to vector controls.miR-181a and miR-182 overexpression resulted in an intense and diffusive pattern of β-galactosidase staining in ULM. |
| Target gene: | AKT3//CCND2 |
| Official symbol(s): | AKT3//CCND2 |
| R-AG-Target gene: | Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot |
| Target gene description: | MiR-181a overexpression can reduce the luciferase activity when transfection of luciferase with AKT3 3 -UTR in myometrial cells Transienttransfection of miR-181a in MM and ULM cells consistently reduced AKT3 expression.MiR-182 overexpression consistently represses CCND2 expression in all MM and LM cells tested. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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