| Gene name: | GATA4 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Brain |
| Cell name: | IMR-90,BJ |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | Ectopic expression of GATA4 induced senescence in human foreskin fibroblasts and IMR-90 fibroblasts,as shown by increased senescence-associated β-galactosidase (SA-β-Gal) activity and decreased 5-bromo-2′-deoxyuridine (BrdU) incorporation. |
| Target gene: | TRAF3IP2//IL1A |
| Official symbol(s): | TRAF3IP2//IL1A |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//qRT-PCR |
| Target gene description: | TRAF3IP2 depletion partially blocked GATA4 activation of NF-kB, as assessed by the expression of SASP genes.Furthermore, ectopic expression of TRAF3IP2 partially rescued the reduced SASP caused by GATA4 depletion in IR-induced senescent cells.Depletion of IL1A reduced GATA4 activation of NF-kB, as assessed by the expression of SASP genes. Furthermore, activation of SASP-associated genes was transmitted to cells lacking GATA4 induction by conditioned medium from GATA4-induced cells.Thus, GATA4 appears to act, at least in part, through TRAF3IP2 and IL1A to activate NF-kB in activating the SASP. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | GATA4 expression triggered NF-kB activation, and GATA4 depletion inhibited NF-kB activation during senescence . |
Annotation:
Loading,please wait...