| Gene name: | P3H1 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | Knockdown//SA-β-gal activity assay//Colony formation assay//Cell proliferation assay |
| Description: | The down-regulation of LEPRE1, LIMA1, MAGOHA, and MAGOHB caused a significant reduction in cell growth (p ≤ 0.05) at 4 days after transfection, when compared with a negative siRNA control. In parallel, we tested if knockdown of the nine genes also induces the SA-β-gal activity.SiRNAmediated down-regulation of LEPRE1, LIMA1, and MAGOHA and MAGOHB robustly increased the number of SA-β-gal-positive cells, compared to cells transfected with siRNA control.a clonogenic test performed on young IMR90 cells after the inhibition of LEPRE1, LIMA1, MAGOHA or MAGOHB by the corresponding siRNAs demonstrated that LEPRE1 or LIMA1 down-regulation strongly inhibited the formation of colonies, compared with a negative siRNA control, while MAGOHA or MAGOHB down-regulation hampered treated cells from resuming proliferation. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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