| Gene name: | PRKCH |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | Knockdown//SA-β-gal activity assay//Cell proliferation assay//Colony formation assay//Western blot |
| Description: | In response to H2O2 or etoposide, PKCη-knockdown cells showed significantly and reproducibly lower number of SA-β-galactosidase-stained cells compared with scrambled control cells.A lower number of proliferating cells were regrown out of senescent cultures of scrambled (sh scr 5-3) control cells compared with PKCη-knockdown (sh 3-3 and sh 2-2) cells in colony formation assays. Viability assays (Neutral Red) with additional PKCη-knockdown clones (sh 4-2 and sh 3-5) showed similar results (data not shown).p21Cip1 is required to induce senescence. Our results showed increased expression of p21Cip1 and p27Kip1 in PKCηexpressing cells (sh scr 5-3), which was markedly reduced in PKCη-knockdown cells upon etoposide treatment. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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