| Gene name: | SELENOH |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | Knockdown//SA-β-gal activity assay//Cell proliferation assay |
| Description: | Moreover, the complete growth inhibition of SelH shRNA MRC5 cells maintained under 20% O2 for 5 weeks could be partially rescued when grown in a 3% O2 incubator, although SelH shRNA MRC-5 cells remained to proliferate poorly (~300-fold slower) as compared to scrambled shRNA MRC-5 cells.the percent SA-β-gal positive cells 5 days after recovery were 17% and 70% in scrambled shRNA and SelH shRNA MRC-5 cells, respectively, in a 20% O2 incubator. When maintained under 3% O2, they dropped (p < 0.05) to 2% and 34 % in scrambled and SelH shRNA MRC-5 cells, respectively. |
| Regulatory pathway: | ATM-P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | ATM-TP53 |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Proliferation assay |
| Pathway description: | Treatment with Ku 60019 (5 μM), a specific ATM kinase inhibitor (53), for 28 days alleviated the slow proliferation phenotype by 3- fold in SelH shRNA MRC-5 cells, but slightly suppressed the proliferation in scrambled shRNA MRC-5 cells.Analyses of SA-βgal staining confirmed that the ATM kinase is required to senesce SelH shRNA cells (40% vs 5%) after being cultured for 28 days. SelH shRNA MRC-5 cells significantly accumulated (p < 0.05) additional H2AX and pATM Ser-1981 after 28 days in a 20% O2 incubator, but such induction was completely. |
Annotation:
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