| Gene name: | DHX9 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | Knockdown//SA-β-gal activity assay//Flow cytometry//Growth curve assay |
| Description: | DHX9-suppressed cells stained positive for SA β-gal, a distinguishing marker of senescent cells.DHX9 shRNA-expressing cells show a significantly higher percentage of SAβ -gal-positive cells compared with MRC-5 controls (50–57% for DHX9 knockdown cells compared with 3% for shFLuc.1309-expressing cells). Measurements of the growth rates of DHX9-depleted MRC-5 cells indicated these to be at least 2-fold lower than control cells and similar to hRAS V12-expressing cells . Cell cycle analyses showed an increase in the percentage of cells in the G0/G1 phase and a decrease in the percentage of cells in the S and G2 phases 14 days after infection of DHX9 shRNAs. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//SA-β-gal activity assay//Western blot |
| Pathway description: | Loss of p53 completely abolished the SA-β-gal staining and rescued the growth defect in DHX9 knockdown cells. In addition, loss of p53 eradicates the p21 and RB1 responses in the DHX9 knockdown cells, compared with the vector control .This demonstrates that p53 is essential for DHX9-induced senescence. |
Annotation:
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