| Gene name: | LGALS3 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Stomach |
| Cell name: | AGS,MEF |
| Gene ID: | 404021 |
| Category: | protein coding |
| Phenotype: | Retinoblastoma |
| Experimental category: | HL |
| PMID: | 24971481 |
| Experiment: | Knockdown//SA-β-gal activity assay//Flow cytometry//Cell proliferation assay |
| Description: | Treatment of AGS cells with 10 mM galectin-3 siRNA (Gal3 siRNA) for 2 days reduced cell proliferation by 50%.The depletion of galectin-3 significantly increased the number of G1 cell cycle-arrested cells and the sub-G1 populations, whereas co-depletion of galectin-3 and p27KIP1 produced equivalent proliferation to scRNA control group cell populations.Galectin-3 -/- MEFs showed growth retardation when compared with galectin-3 +/+ MEFs.Depletion of galectin-3 resulted in growth retardation and premature senescence in the human foreskin fibroblasts. |
| Target gene: | P27 |
| Official symbol(s): | P27 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//RT-PCR//SA-β-gal activity assay//Cell proliferation assay |
| Target gene description: | Galectin-3 depletion was accompanied by decreased Skp2 and increased p27KIP1 expression. Although the expression of p16INK4A and p21WAF1/CIP1 remained unchanged, p14ARF levels were found to decrease slightly.Galectin-3 depletion reduced Skp2 mRNA but increased p27KIP1 protein expression without a change in the mRNA expression. This suggests that galectin-3 regulates the level of p27KIP1 protein by Skp2-mediated ubiquitin activity.Upon galectin-3 depletion, both the cell types exhibited growth retardation and additional p27KIP1 ablation reversed this growth retardation . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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