| Gene name: | ERRFI1 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Gene ID: | 54206 |
| Category: | protein coding |
| Phenotype: | Retinoblastoma |
| Experimental category: | L |
| PMID: | 24815188 |
| Experiment: | SA-β-gal activity assay |
| Description: | Myc–Mig-6 overexpression increased cell senescence as evidenced by the elevated number of the SA-?-gal positive, blue coloured cells compared to vector control transfected fibroblasts. |
| Regulatory pathway: | ERK//AKT |
| R-AG-Pathway: | Activation//Activation |
| Official symbol(s): | MAPK1// |
| Pathway experiment: | Western blot |
| Pathway description: | The strong signal for phosphorylated EGFR (pEGFR Tyr1173) observed in early passage cells at 5 min of EGF stimulation indicated the quick and transient response . Late passage (P25) fibroblasts exhibited an attenuated acute response to EGF treatment as evidenced by the slightly decreased levels of Tyr1173 phosphorylated EGFR at 5 min.Downstream of the EGFR, ERK activity was sustained in late passage as compared to early passage cells, whereas AKT activity was massively down-regulated in late passage cells . Moreover, the expression level of p16INK4A, a typical senescence-associated protein, was generally elevated in late passage cells as compared to young fibroblasts. |
Annotation:
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