| Gene name: | SOX2 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Tumor tissue |
| Cell name: | HCT116 |
| Gene ID: | 6657 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 23451179 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell proliferation assay//Western blot |
| Description: | Our cell proliferation and cell cycle analyses indicated that Sox2 infection into HCT116 cells suppressed proliferation by inducing accumulation of cells in G0/G1 and sub-G1 and reduction of S cell cycle phases compared with mock-infected cells. The attenuation of cell proliferation was associated with about 90% inhibition of anchorage-independent cancer growth in soft agar, a hallmark of cancer cell properties.We then examined senescence using an SA-?galactosidase assay and found that ectopic expression of Sox2 enhanced ?-galactosidase activity and protein level and suppressed Ki-67 protein level, a cell proliferation marker. |
| Target gene: | ATG10 |
| Official symbol(s): | ATG10 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Luciferase reporter assay//Western blot//RT-PCR//Immunochemical staining |
| Target gene description: | Our cycledependent RT-PCR results demonstrated that the ATG10 mRNA level from Sox2-transfected cells was increased by about 2.5 fold compared with mock.Notably, Western blotting results and immunocytofluorescence against ATG10 indicated that ATG10 and LC3 protein levels were increased by overexpression of Sox2.We found that that deletion of putative Sox2 binding region significantly suppressed luciferase activity. Notably, ATG10 promoter activity was increased by overexpression of Sox2. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...