| Gene name: | ZMAT3 |
| Aging type: | Prevent |
| Aging characteristic: | Others |
| Tissue type: | Lung |
| Cell name: | MCF-7 |
| Experiment: | Knockdown//SA-β-gal activity assay//Flow cytometry//Colony formation assay//Cell proliferation assay |
| Description: | MCF7 breast cancer cells, which were treated with an siRNA against Wig1 (Wig Si), exhibited decreased cell proliferation and clonogenic ability.Interestingly, Wig1 depletion induced typical senescence associated phenotypic changes: large and flattened morphology, positive senescence-associated β-galactosidase (SA-βGal) staining, and G1 cell-cycle arrest. No significant increase in the subG1 phase was observed. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot//Knockdown |
| Target gene description: | Overexpression and depletion of Wig 1 downregulated and upregulated p21, respectively, at both the mRNA and protein levels.The qRT–PCR assay revealed that Wig1-wt and Wig1-mut1 reverted the p21 3' UTR reporter mRNA levels that were increased due to Wig1 depletion to levels similar to those of Con Si-transfected cells, whereas Flag–Wig1- mut2 failed to rescue these reporter mRNA levels. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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