| Gene name: | NEK4 |
| Aging type: | Accelerate |
| Aging characteristic: | Others |
| Tissue type: | Foreskin |
| Cell name: | HFF,BJ,293T,IMR-90,WI-38 |
| Experiment: | Knockdown//SA-β-gal activity assay//BrdU assay//RT-PCR |
| Description: | Long-term culture of BJ cells in which NEK4 expression is suppressed (BJ shN4) revealed an extension of the time to replicative senescence of 10 to 20 PD compared to BJ shGFP cells.At late passage (PD 55 to 60), BJ shN4 cells proliferated at nearly twice the rate of BJ shGFP cells .Suppression of NEK4 by shN4 no. 1 resulted in a decrease in p21 mRNA levels smaller than that observed upon expression of shN4 no. 2 (0.81- and 0.27-fold, respectively), but we note that the levels of p21 mRNA and protein were still reduced compared to those in BJ shGFP cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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